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enzyme) from the adjacent subunit ion-pairs Along with the phosphate moiety on the substrate (ten). It is actually unlikely this arginine residue could add to the diminution in k
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m though the slot naga26 Zn enzyme has the lowest. Identical traits inside the range of the catalytic constants had been noted Formerly for the Co, Zn, Mn and Fe reconstitutions of apo-NagA from E. coli
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Incubation of your apo-enzyme with a person equivalent of Zn2+ for thirty minutes thoroughly restored catalytic exercise. The titration of apo-enzyme with various quantities of ZnCl2 is revealed in Determine one. These benefits demonstrate that NagA from E. coli